1,718 followers
PROTOCOL Introducing Large Genomic Deletions in Human Pluripotent Stem Cells Using CRISPR-Cas3. Hou Z et al. Curr Protoc. 2022-02-07. https://t.co/fZOkDVqTlD ... purifying the Type I-E CRISPR proteins Cascade & Cas3 from T. fusca, electroporating RNP